Proteintech, 86565-1-RR, CCL12/MCP-5 Recombinant monoclonal antibody

Size: 20ul / 100ul The CCL12/MCP-5 (86565-1-RR) by Proteintech is a Recombinant antibody targeting CCL12/MCP-5 in WB, IF/ICC, ELISA applications with reactivity to mouse samples 86565-1-RR targets CCL12/MCP-5 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: Transfected with Mouse Ccl12 Transfected HEK-293T cells Positive IF/ICC detected in: Transfected HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information CCL12 (monocyte chemotactic protein-5, MCP-5) is a murine CC-chemokine encoded within the chemokine cluster on chromosome 11. It is the closest murine homologue of human MCP-1 (CCL2), sharing 66 % amino-acid identity, and signals exclusively through the G-protein-coupled receptor CCR2. CCL12 is produced by stromal cells, astrocytes, macrophages and, under inflammatory conditions, by lung and bone-marrow stromal cells. Its receptor, CCR2, is expressed on monocytes, memory T cells, NK cells, dendritic cells and basophils. Consequently, CCL12 potently chemoattracts monocytes, lymphocytes, eosinophils and bone-marrow-derived fibrocytes, but not neutrophils. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg3315 Product name: Recombinant Mouse CCL12/MCP-5 protein (rFc Tag) (HPLC verified) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-104 aa of NM_011331.2 Sequence: GPDAVSTPVTCCYNVVKQKIHVRKLKSYRRITSSQCPREAVIFRTILDKEICADPKEKWVKNSINHLDKTSQTFILEPSCLG Predict reactive species Full Name: chemokine (C-C motif) ligand 12 Calculated Molecular Weight: 12KD GenBank Accession Number: NM_011331.2 Gene Symbol: Ccl12 Gene ID (NCBI): 20293 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q62401 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.