Proteintech, 86612-3-RR, C9orf78 Recombinant monoclonal antibody

Size: 20ul / 100ul The C9orf78 (86612-3-RR) by Proteintech is a Recombinant antibody targeting C9orf78 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86612-3-RR targets C9orf78 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, RAW 264.7 cells, Neuro-2a cells, NIH/3T3 cells, PC-12 cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information The C9orf72 protein is highly expressed in neurons and microglial cells. Its precise function has not been fully elucidated, but research indicates that it plays an important role in key intracellular pathways such as endosomal vesicle trafficking and autophagy. This protein has garnered significant attention due to its strong association with neurodegenerative diseases. An abnormal expansion of a GGGGCC hexanucleotide repeat sequence in the C9orf72 gene is the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. This expansion may lead to multiple pathogenic mechanisms, including loss of function (haploinsufficiency) and gain of toxicity (such as the production of toxic dipeptide repeat proteins), ultimately resulting in neuronal death. Consequently, C9orf72 has become a critical research target in the field of neuroscience. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5919 Product name: Recombinant human C9orf78 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-289 aa of BC007664 Sequence: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKERGKDKISEEEDLHLGTSFSAETNRRDEDADMMKYIETELKKRKGIVEHEEQKVKPKNAEDCLYELPENIRVSSAKKTEEMLSNQMLSGIPEVDLGIDAKIKNIISTEDAKARLLAEQQNKKKDSETSFVPTNMAVNYVQHNRFYHEELNAPIRRNKEEPKARPLRVGDTEKPEPERSPPNRKRPANEKATDDYHYEKFKKMNRRY Predict reactive species Full Name: chromosome 9 open reading frame 78 Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC007664 Gene Symbol: C9orf78 Gene ID (NCBI): 51759 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NZ63 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.