Proteintech, 86641-1-RR, Phospho-STAT6 (Tyr641) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT6 (Tyr641) (86641-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-STAT6 (Tyr641) in WB, FC (Intra), ELISA applications with reactivity to human samples 86641-1-RR targets Phospho-STAT6 (Tyr641) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IL-4 treated ACHN cells Positive FC (Intra) detected in: IL-4 treated ACHN cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. p-STAT6(Tyr641) is a biomarker of Th2-high inflammation, targeted by IL-4Rα blockers (dupilumab) and JAK1/3 inhibitors. High p-STAT6 in TAMs correlates with M2 polarization and radioresistance, blocking IL-4 or JAK1/3 decreases Tyr641 phosphorylation and restores anti-tumor immunity. (PMID: 37474750) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 6, interleukin-4 induced Calculated Molecular Weight: 100 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: BC005823 Gene Symbol: STAT6 Gene ID (NCBI): 6778 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P42226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.