Proteintech, 86728-1-RR, TRIM47 Recombinant monoclonal antibody

Size: 20ul / 100ul The TRIM47 (86728-1-RR) by Proteintech is a Recombinant antibody targeting TRIM47 in WB, ELISA applications with reactivity to human, mouse, rat samples 86728-1-RR targets TRIM47 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse colon tissue, OVCAR-3 cells, U2OS cells, SMMC-7721 cells, PC-3 cells, rat colon tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis. TRIM47, defined as E3 ubiquitin ligases, physically binds to SMAD4 and enhances the ubiquitination and degradation of SMAD4 (PMID: 39117713, 35513381). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag22868 Product name: Recombinant human TRIM47 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC017299 Sequence: MDELGAGIAQSRRTVALIKSAAVAERERVSRLFADAAAALQGFQTQVLGFIEEGEAAMLGRSQGDLRRQEEQRSRLSRARQNLSQVPEADSVSFLQELLALRLALEDGCGPGPGPPRELSFTKSSQAVRAVRDMLAVACVNQWEQLRGPGGNEDGPQKLDSEADAEPQDLESTNLLESEAPRDYFLKFAYIVDLDSDTADKFLQLFGTKGVKRVLCPINYPLSPTRFTHCEQVLGEGALDRGTYYWEVEIIEGWVSMGVMAEDFSPQEPYDRGRLGRNAHSCCLQWNGRSFSVWFHGLEAPLPHPFSPTVGVCLEYADRALAFYAVRDGKMSLLRRLKASRPRRGGIPASPIDPFQSRLDSHFAGLFTHRLKPAFFLESVDAHLQIGPLKKSCISVLKRR Predict reactive species Full Name: tripartite motif-containing 47 Calculated Molecular Weight: 638 aa, 70 kDa Observed Molecular Weight: 70-75 kDa GenBank Accession Number: BC017299 Gene Symbol: TRIM47 Gene ID (NCBI): 91107 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96LD4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.