Proteintech, 86859-3-RR, Cyclin T2 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cyclin T2 (86859-3-RR) by Proteintech is a Recombinant antibody targeting Cyclin T2 in WB, ELISA applications with reactivity to human samples 86859-3-RR targets Cyclin T2 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, A431 cells, Jurkat cells, HepG2 cells, HuH-7 cells, HT-1080 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Cyclin T2 (CCNT2) belongs to the cyclin family. CCNT2 is regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II)  (PMID: 9499409, PMID: 15563843). Cyclin T2 is expressed at a relatively high level in freshly isolated monocytes and its level remains constant when the cells are induced to undergo macrophage differentiation (PMID: 21791050). In contrast, Cyclin T1 is expressed at low levels in monocytes and it is strongly up-regulated by a post-transcriptional mechanism when the cells are induced to differentiate to macrophages. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16514 Product name: Recombinant human CCNT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 251-610 aa of BC114366 Sequence: LKKIRNWRANQAARKPKVDGQVSETPLLGSSLVQNSILVDSVTGVPTNPSFQKPSTSAFPAPVPLNSGNISVQDSHTSDNLSMLATGMPSTSYGLSSHQEWPQHQDSARTEQLYSQKQETSLSGSQYNINFQQGPSISLHSGLHHRPDKISDHSSVKQEYTHKAGSSKHHGPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIANTEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPHISRDHKEKHKEHPSSRHHTSSHKHSHSHSGSSSGGSKHSADGIPPTVLRSPVGLSSDGI Predict reactive species Full Name: cyclin T2 Calculated Molecular Weight: 730 aa, 81 kDa Observed Molecular Weight: 70-74 kDa GenBank Accession Number: BC114366 Gene Symbol: Cyclin T2 Gene ID (NCBI): 905 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60583 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.