Proteintech, 86875-3-RR, SOLO/SESTD1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SOLO/SESTD1 (86875-3-RR) by Proteintech is a Recombinant antibody targeting SOLO/SESTD1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 86875-3-RR targets SOLO/SESTD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, Jurkat cells, L02 cells, MCF-7 cells, MDCK cells, mouse brain tissue, rat brain tissue Positive IF/ICC detected in: HepG2 cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SEC14 domain and spectrin repeat-containing protein 1 (SESTD1) is also named Huntingtin-interacting protein-like protein and SOLO. SESTD1, a previously uncharacterized lipid-binding protein strongly expressed in aorta, was found to interact with both TRPC proteins and modulate receptor-activated TRPC5 currents in a recombinant cell line (PMID: 20164195). SESTD1 is proposed to be involved in phospholipid regulation of transient receptor potential channels TRPC4 and TRPC5 (PMID: 26503763). SESTD1 expression is associated with high abundance of immune infiltration, including T helper cells, Th2 cells, and Macrophages in HCC (PMID: 41016345). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag21016 Product name: Recombinant human SESTD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 348-696 aa of BC047578 Sequence: DLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQQLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVDVGLQGLREKGQGLLDQISNQASWAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQAVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLCQSLRCTSRSSGDTLPRLNRVWKQFTIASEERVHRLEMAIAFHSNAEKILQDCPEEPEAINDEEQFDEIEAVGKSLLDRLTVPVVYPDGTEQYFGSPSDMASTAENIRDRMKLVNLKRQQLRHPEMVTTES Predict reactive species Full Name: SEC14 and spectrin domains 1 Calculated Molecular Weight: 696 aa, 79 kDa Observed Molecular Weight: 79 kDa GenBank Accession Number: BC047578 Gene Symbol: SOLO Gene ID (NCBI): 91404 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q86VW0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.