Proteintech, 86946-1-RR, EMB Recombinant monoclonal antibody

Size: 20ul / 100ul The EMB (86946-1-RR) by Proteintech is a Recombinant antibody targeting EMB in WB, IF/ICC, ELISA applications with reactivity to human samples 86946-1-RR targets EMB in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, Jurkat cells, MCF-7 cells, LNCaP cells Positive IF/ICC detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycosylated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg6407 Product name: Recombinant human EMB protein Source: mammalian cells -derived, V37 Tag: C-rFc Domain: 33-260 aa of NM_198449.3 Sequence: DGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELVVLSYLVP Predict reactive species Full Name: embigin homolog (mouse) Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 60-75 kDa GenBank Accession Number: NM_198449.3 Gene Symbol: EMB Gene ID (NCBI): 133418 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q6PCB8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.