Proteintech, 86990-2-RR, Di-Methyl-Histone H3 (Lys9) Recombinant monoclonal antibody

Size: 20ul / 100ul The Di-Methyl-Histone H3 (Lys9) (86990-2-RR) by Proteintech is a Recombinant antibody targeting Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human samples 86990-2-RR targets Di-Methyl-Histone H3 (Lys9) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293T cells Positive IF/ICC detected in: HeLa cells Positive Dot Blot detected in: / / Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1250-1:5000 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information H3K9me2 is a typical transcriptional repressive modification, primarily enriched in heterochromatin regions (such as centromeres, telomeres, and transposon elements), but can also be scattered throughout euchromatin. By recruiting "reader" proteins such as HP1 (Heterochromatin Protein 1), H3K9me2 can establish and stabilize a chromatin condensation state, thereby silencing gene expression. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15-17 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.