Proteintech, 87153-1-RR, Phospho-CD19 (Tyr531) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-CD19 (Tyr531) (87153-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-CD19 (Tyr531) in WB, ELISA applications with reactivity to human samples 87153-1-RR targets Phospho-CD19 (Tyr531) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Ramos cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. Phospho-CD19 (Tyr531) is a phosphorylated form of CD19, which is a protein involved in B cell receptor (BCR) signaling and plays a crucial role in B cell development and function. Phosphorylation of CD19 at Tyr531 is known to recruit PI3K activity leading to Akt activation and attendant S473 phosphorylation. This activation of BCR induces multiple oncogenic signals and treatment with ibrutinib prevents preferentially the activation of BTK in both patients with ABC-like and GCB-like molecular features. (PMID: 20038598) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: CD19 molecule Calculated Molecular Weight: 556 aa, 61 kDa Observed Molecular Weight: 95 kDa GenBank Accession Number: NM_001770 Gene Symbol: CD19 Gene ID (NCBI): 930 ENSEMBL Gene ID: ENSG00000177455 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P15391 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.