Proteintech, 87337-1-RR, Phospho-CAD (Ser1859) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-CAD (Ser1859) (87337-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-CAD (Ser1859) in WB, ELISA applications with reactivity to human samples 87337-1-RR targets Phospho-CAD (Ser1859) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, λ phosphatase treated HEK-293 cells, λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) is a phosphorylated form of the CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) enzyme at Serine 1859. This site is specifically phosphorylated by mTOR complex 1 (mTORC1), acting through the downstream kinase S6K1, and plays a role in regulating pyrimidine biosynthesis. The phosphorylation of CAD at Ser1859 is a part of the PI3K-AKT-mTORC1-S6K1 pathway that integrates growth signals to regulate cell growth, proliferation, and homeostasis by modulating several anabolic and catabolic processes.In addition, insulin also stimulates the Ser1859 phosphorylation of CAD in a manner sensitive to rapamycin as well as phosphatase and tensin homolog (PTEN) deletion in the PI3K-AKT pathway. (PMID:25422319, PMID: 34638594) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Calculated Molecular Weight: 2225 aa, 243 kDa Observed Molecular Weight: 250 kDa GenBank Accession Number: NM_004341 Gene Symbol: CAD Gene ID (NCBI): 790 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P27708 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.