Proteintech, 87449-1-RR, CD18 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD18 (87449-1-RR) by Proteintech is a Recombinant antibody targeting CD18 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 87449-1-RR targets CD18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, THP-1 cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: THP-1 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during the immune response, and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg10481 Product name: Recombinant human ITB2 protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-Twin-Strep-Tag Domain: 10011434 of Sequence: \ Predict reactive species Full Name: integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) Observed Molecular Weight: 90-100 kDa Gene Symbol: CD18 Gene ID (NCBI): 3689 ENSEMBL Gene ID: ENSG00000160255 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05107 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.