Proteintech, 98262-1-RR, Anti-GGGGS Linker Rabbit Recombinant Antibody

Size: 100ug The GGGGS Linker (98262-1-RR) by Proteintech is a Recombinant antibody targeting GGGGS Linker in WB, IHC, IF/ICC, FC applications with reactivity to recombinant protein samples 98262-1-RR targets GGGGS Linker in WB, IHC, IF/ICC, FC applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: Transfected HEK-293T cells Positive IHC detected in: Transfected HEK-293T cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Transfected HEK-293T cells, Transfected CHO-K1 Positive FC detected in: Transfected HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:40000-1:160000 Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension Background Information As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S*2, G4S*3, G4S*4, and G4S*5. Specification Tested Reactivity: recombinant protein Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GGGGS Linker Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.