Proteintech, 98624-1-RR, Anti-Human Survivin Rabbit Recombinant Antibody

Size: 100ug The Survivin (98624-1-RR) by Proteintech is a Recombinant antibody targeting Survivin in IF/ICC, FC (Intra) applications with reactivity to human samples 98624-1-RR targets Survivin in IF/ICC, FC (Intra) applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: MCF-7 cells, A549 cells Positive FC (Intra) detected in: FC receptor blocked human PBMCs Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg4645 Product name: Recombinant human Survivin protein Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 1-142 aa of / Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD Predict reactive species Full Name: baculoviral IAP repeat-containing 5 Calculated Molecular Weight: 16kDa GenBank Accession Number: / Gene Symbol: Survivin Gene ID (NCBI): 332 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15392-1 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.