Proteintech, Biotin-SF98190, Biotin Anti-Human CD98/SLC3A2 Rabbit scFv Antibody

Size: 100ug The CD98/SLC3A2 (Biotin-SF98190) by Proteintech is a Recombinant antibody targeting CD98/SLC3A2 in FC, ELISA applications with reactivity to human samples Biotin-SF98190 targets CD98/SLC3A2 in FC, ELISA applications and shows reactivity with human samples. Tested Applications Positive FC detected in: human PBMCs Recommended dilution Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter, encoded by the SLC7A5) and an 85-kDa glycosylated heavy chain (CD98hc, encoded by the SLC3A2) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289). Specification Tested Reactivity: human Host / Type: Rabbit / scFv Class: Recombinant Type: Single-chain variable fragment antibody (scFv) Immunogen: CatNo: Eg1948 Product name: Recombinant Human CD98/SLC3A2 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 206-630 aa of NM_002394.5 Sequence: RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA Predict reactive species Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 Calculated Molecular Weight: 68 kDa GenBank Accession Number: NM_002394.5 Gene Symbol: CD98 Gene ID (NCBI): 6520 Conjugate: Biotin Excitation/Emission Maxima Wavelengths: - Source: Anti-Human CD98/SLC3A2 scFv from clone 241755D6, consisting of VH-GGGGS linker-VL (N- to C-terminus), with His tag at the C-terminus. Form: Liquid Purification Method: Affinity purification Storage Buffer: PBS with 4% sucrose and 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.