Product Description
Size: 100ug
The CD147 (Biotin-SF98229) by Proteintech is a Recombinant antibody targeting CD147 in FC, ELISA applications with reactivity to mouse samples
Biotin-SF98229 targets CD147 in FC, ELISA applications and shows reactivity with mouse samples.
Tested Applications
Positive FC detected in: mouse thymocytes
Recommended dilution
Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Background Information
CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211).
Specification
Tested Reactivity: mouse
Host / Type: Rabbit / scFv
Class: Recombinant
Type: Single-chain variable fragment antibody (scFv)
Immunogen: CatNo: Eg1530 Product name: Recombinant Mouse CD147 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 22-325 aa of NM_009768.2 Sequence: AAGFLKAPLSQERWAGGSVVLHCEAVGSPIPEIQWWFEGNAPNDSCSQLWDGARLDRVHIHAAYRQHAASSLSVDGLTAEDTGTYECRASSDPDRNHLTRPPRVKWVRAQASVVVLEPGTIQTSVQEVNSKTQLTCSLNSSGVDIVGHRWMRGGKVLQEDTLPDLHTKYIVDADDRSGEYSCIFLPEPVGRSEINVEGPPRIKVGKKSEHSSEGELAKLVCKSDASYPPITDWFWFKTSDTGEEEAITNSTEANGKYVVVSTPEKSQLTISNLDVNVDPGTYVCNATNAQGTTRETISLRVRSR Predict reactive species
Full Name: basigin
Calculated Molecular Weight: 42 kDa
GenBank Accession Number: NM_009768.2
Gene Symbol: CD147
Gene ID (NCBI): 12215
Conjugate: Biotin
Excitation/Emission Maxima Wavelengths: -
Source: Anti-Mouse CD147 scFv from clone 241852B4, consisting of VH-GGGGS linker-VL (N- to C-terminus), with His tag at the C-terminus.
Form: Liquid
Purification Method: Affinity purification
Storage Buffer: PBS with 4% sucrose and 0.09% sodium azide, pH 7.3.
Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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