Qiagen, 330171, Custom RT2 PCR Array

96-well plate, 384-well plate, or Rotor-Disc 100 including assays, film, or cap strips

Features

- Robust and reliable biomarker validation
- Flexible array format for over 100,000 genes
- Rigorous QC and QA process for accurate and reliable results
- Designed for routine use on any real-time cycler
- Ideal for verification of microarray or RNAseq results

Performance

Sensitivity

Reproducibility

Specificity

Uniform PCR amplification efficiency

Principle

Format: Number of plates (minimum)
8 genes, 12 samples per plate: 12
12 genes, 8 samples per plate: 12
16 genes, 6 samples per plate: 12
24 genes, 4 samples per plate: 24
32 genes, 3 samples per plate: 24
48 genes, 2 samples per plate: 24
96 genes, 1 sample per plate: 24
All formats: Per additional 12 array

Format: Number of plates (minimum)
8 genes, 48 samples per plate: 6
12 genes, 32 samples per plate: 6
24 genes, 16 samples per plate: 6
32 genes, 12 samples per plate: 6
48 genes, 8 samples per plate: 6
64 genes, 6 samples per plate: 6
96 genes, 4 samples per plate: 6
128 genes, 3 samples per plate: 6
192 genes, 2 samples per plate: 24
384 genes, 1 sample per plate: 24
All formats: Per additional 6 array

Custom RT 2 Profiler PCR Arrays are reliable tools for studying and verifying gene expression biomarkers in large numbers of biological samples. Whether the biomarkers are from a proprietary collection, literature research or DNA microarray analysis, QIAGEN’s expertise in real-time PCR detection provides quality, flexibility and consistency in biomarker validation experiments. QIAGEN’s genomewide coverage provides primers for over 100,000 genes covering the human, mouse, rat, Rhesus macaque, drosophila and dog genomes. SYBR Green-based real-time PCR is a powerful technology for studying and validating gene expression biomarkers in large numbers of biological samples. Each 96-well plate, 384-well plate or 100-well disc PCR array includes SYBR Green-optimized primer assays. The high-quality primer design and master mix formulation enable the PCR array to amplify gene-specific products simultaneously under uniform cycling conditions. Rigorous and robust quality assurance (QA) and quality control (QC) procedures covering primer design and quality, specificity and amplification efficiency provide peace of mind. This combination provides Custom RT 2 Profiler PCR Arrays with the specificity and the high amplification efficiencies required for accurate real-time SYBR Green results. PCR arrays are easy to use in any research laboratory.

Available formats

Custom RT2 Profiler PCR Arrays are available in the following plate layouts (see the tables below and figure " Available plate layouts ". Select the genes and the plate format required.

Arrays are available in additional formats or with special assay designs (position, variants, etc.), please inquire.

Procedure

- Data normalization
- Genomic DNA contamination detection
- RNA sample quality
- General PCR performance
- Easy-to-use data analysis

Simply mix the cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program (see flowchart " Simple procedure "). Custom RT 2 Profiler PCR Arrays are compatible with all QIAGEN, ABI, Bio-Rad, Eppendorf, Roche and Stratagene instruments.

Flexible layout and controls

Custom RT 2 Profiler PCR Arrays are available in 96-well plate, 384-well plate and 100-well disc formats, and are used to monitor the expression of up to 84 or 370 genes related to a specific research area of interest, plus 5 housekeeping genes. Custom RT 2 Profiler PCR Arrays may also include control elements for:

Data can be analyzed using an easy-to-use Excel-based data analysis template or web-based software. Data analysis is based on the ΔΔC T method with normalization of the raw data to either housekeeping genes.

Applications

- Cancer
- Inflammation and cytokine profiling
- Neuroscience
- Signal transduction pathways
- Cell adhesion and cell migration

Custom or Modified RT 2 PCR Profiler Arrays offer a robust platform for biomarker screening and validation and can be used in all areas of biological and medical research, including: