Revvity, 62HTGFBPEG, HTRF Human and Mouse TGF-β1 Detection Kit, 500 Assay Points

Overview
TGF beta 1 (Transforming Growth Factor Beta-1) is considered one of major cytokines involved in regulation of extracellular matrix (ECM) synsis and degradation, as well as a major profibrotic factor. TGF beta 1 pre-proprotein is processed to generate a latency-associated peptide (LAP) and a mature TGFß1 peptide, which remain associated through strong non-covalent interactions. An activation step (MMP2 cleavage or acid activation) is required to release active form of TGF beta 1 from TGFß1-LAP complex.
Assessment of serum samples often requires enhanced sensitivity. In some cases, AlphaLISA assays may have sufficient sensitivity to enable detection of low levels of analytes in serum or plasma. When assaying, always follow recommended protocol and avoid highly haemolyzed samples.
How it works
Activation step
The samples and cell culture supernatants contain TGF beta 1 and a TGFß1-LAP complex, which require an acid activation step followed by a neutralization in order to be detected (sample activation can follow one of the protocols described on the right). The reagents needed for this activation step are provided in the kit for maximum convenience. Standards and activated samples are dispensed directly into the detection (white, low-volume) plate for the detection by HTRF® reagents.
 

Assay principle
Cell supernatant, sample, or standard is dispensed directly into the assay plate for the detection by HTRF® reagents (384-well low-volume white plate or Revvity low-volume 96-well plate in 20 µl). The antibodies labeled with the HTRF donor and acceptor are pre-mixed and added in a single dispensing step, to further streamline the assay procedure. The assay can be run up to a 1536 well-format by simply resizing each addition volume proportionally. Application-Protein Quantification
Brand-HTRF
Detection Modality-HTRF
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Cytokines
Target Species-Human Mouse
Technology-TR-FRET
Therapeutic Area-Metabolism/Diabetes NASH/Fibrosis Oncology & Inflammation
Unit Size-500 assay points