Revvity, 64TOM20PEG, HTRF Human and Mouse TOM20 Detection Kit, 500 Assay Points

Overview
The removal of damaged mitochondria is critical for maintaining cellular homeostasis. The Translocase of Outer Membrane (TOM) receptor complex is responsible for the movement of proteins through the outer mitochondrial membrane of the mitochondria into the intermembrane space. TOM20 is a central component of the TOM complex. Monitoring the degradation of TOM20 is an indicator of mitophagy activation. This kit is intended to measure TOM20 in cellular lysates. The assay is a fast alternative to ELISA, thanks to the easy implementation of our Add and Read method.
How it works
Assay principle
TOM20 is measured using a sandwich immunoassay involving two specific anti-TOM20 antibodies, respectively labeled with HTRF donor and acceptor dyes. The intensity of the signal is directly proportional to the concentration of TOM20 present in the sample.

Assay Protocol
The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of TOM20 HTRF detection reagents. This protocol enables the cells' viability and confluence to be monitored. Application-Protein Quantification
Automation Compatible-Yes
Brand-HTRF
Detection Modality-HTRF
Lysis Buffer Compatibility-Lysis Buffer 1 Lysis Buffer 4
Product Group-Kit
Sample Volume-16 µL
Shipping Conditions-Shipped in Dry Ice
Target Class-Biomarkers
Target Species-Human Mouse
Technology-TR-FRET
Therapeutic Area-Oncology & Inflammation
Unit Size-500 assay points