Revvity, TRF1209M, LANCE Human ADIPONECTIN Detection Kit, 10,000 Assay Points

Overview
The LANCE® Ultra Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Adiponectin (Acrp30, in mouse) is a hormone secreted by differentiated adipocytes that regulates energy homeostasis and glucose and lipid metabolism. It is expressed as a ~35 kDa protein. It has been demonstrated that Adiponectin / Acrp30 plays a major role in controlling whole-body metabolism, particularly by enhancing insulin sensitivity in muscle and liver, and by augmenting the oxydation of fatty acids in muscle. Application-Protein Analysis & Detection
Assay Points-10000
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-TR-FRET
Format-Microplates
Target-Adiponectin
Target Class-Protein
Therapeutic Area-Metabolic
Unit Size-10,000 Assay Points