Revvity, TRF1294C, LANCE Human Albumin Detection Kit, 500 Assay Points

Overview
The LANCE® Ultra Human Albumin Detection Kit is designed for detection and quantitation of human albumin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

No-wash steps, no separation steps
TR-FRET technology
Sensitive detection
High reproducibility
Faster time-to-results
Easy automation
96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Human serum albumin (HSA) is a 66 kDa protein produced by the liver. HSA constitutes two thirds of the protein mass of serum and plays a key role in the transport of small molecules in the blood such as fatty acids, vitamins, hormones, divalent cations, and drugs. Another important function of HSA is to maintain osmotic pressure and pH in the blood. HSA is an important biomarker in kidney disease. In albuminuria, albumin is not reabsorbed properly by kidney glomeruli and is excreted in the urine. Generally, in hypoalbuminemia, a lower level of albumin in serum can be explained by a kidney disease, poor liver function, or inflammation. Application-Protein Quantification
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Dynamic Range-68.1- 100,000 pg/mL
Limit of Detection-68.1 pg/mL
Limit of Quantification-369.2 pg/mL
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-Albumin
Target Class-Biologics
Target Species-Human
Technology-TR-FRET
Therapeutic Area-Metabolic
Unit Size-500 assay points