Thermo Fisher, 01-2222-42, UltraComp eBeads™ Compensation Beads Invitrogen™

UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. They are designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.

Each drop of beads contains two populations: a positive population that will capture any mouse, rat, or hamster antibody and a negative population that will not react with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations result. This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments.

UltraComp eBeads are compatible with all fluorochromes excited by an ultraviolet (355 nm) or violet (405 nm) laser. They should be used with standard staining buffers which contain PBS or HBSS, protein such as BSA or FBS, and sodium azide. No other additives should be used. For more information, please contact technical support.

Reactivity/Species
Hamster, Mouse, Rat

Reported Application
Flow Cytometric Analysis

FAQs

1. How long are UltraComp eBeads Compensation Beads (Cat. No. 01-2222-41, 01-2222-42) stable after staining?

After staining, UltraComp eBeads Compensation Beads (Cat. No. 01-2222-41, 01-2222-42) are stable for up to 3 days if the samples are stored in a fixative. If the samples are stored in FACS buffer, they are stable for 1 week.

2. I am performing flow cytometry. My compensation matrix has values over 100. That's not possible is it?

It is possible. But if you have a lot of values over 100, you probably need to look at the voltages that you are using for your data collection.

3. What kind of controls do I need for flow cytometry?

For compensation, you need to prepare a singly stained sample (or compensation beads) for each color parameter that you are using. In addition, we recommend that you use FMO (flow minus one) controls. These are controls in which you label cells or beads with every color in your panel, omitting one. Make one FMO control for each color. These controls are important for helping you properly set gates on your data.