Thermo Fisher, K430001, pBAD TOPO™ TA Expression Kit

His Tag (6x), 20 Reactions The pBAD TOPO™ TA Expression Kit is specifically designed for one-step cloning and regulated prokaryotic expression ofTaq-amplified PCR products. Once you've TOPO™ Cloned your PCR product, you can go straight to protein expression. Some of the convenient features of the pBAD-TOPO™ vector include:
• Linearized, topoisomerase I-activated vector for 5-minute cloning ofTaqamplified PCR products
• ThearaBADpromoter for tightly regulated expression inE. coliV5 epitope tag for detection with an Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptideAntibiotic Resistance Bacterial: Ampicillin (AmpR)
Bacterial or Yeast Strain: LMG194, TOP10
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Expression Mechanism: Cell-Based Expression
Expression System: E. coli
Inducing Agent: Arabinose
Product Type: TA Expression Kit
Quantity: 20 Reactions
Selection Agent (Eukaryotic): None
Vector: pBAD
Cloning Method: TOPO-TA
Product Line: TOPO™
Promoter: araBAD
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each