Thermo Fisher, PV6087, Kinase Tracer 314, 25 μM

Kinase Tracer 314, 25 μM In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or “tracer” such as Kinase Tracer 314 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
Learn More and See Which Kinases Have Been Validated Using This AssayAssay: Kinase Binding Assay
Assay Entry: Biochemical binding/kinetic
Excitation/Emission: 340/615, 665
For Use With (Application): TR-FRET
For Use With (Equipment): Microplate Reader
Format: 384-well Plate
Label or Dye: Alexa Fluor™ 647
Quantity: 25 μL
Readout: Kinetic
Shipping Condition: Dry Ice
Target Entry: PIK3CG, PIK3CD/PIK3R1, mTOR, FRAP1
Validated Kinases: PIK3CD, PIK3CG, FRAP1 (mTOR)
Type: Kinase Tracer
Unit Size: Each