Waters, 186002160, BioSuite Phenyl Column, 1000Å, 13 µm, 21.5 mm X 150 mm, 1/pk

The separation of proteins and peptides based upon hydrophobic characteristics is a powerful chromatographic technique. However, some proteins denature at elevated organic solvent concentrations making reversed-phase chromatography (RPC) difficult. Waters BioSuite™ Phenyl HIC columns provide a viable separation alternative to RPC. HIC is characterized by the adsorption of compounds to a weakly hydrophobic surface at high salt concentrations, followed by elution with a decreasing salt gradient. HIC combines the non-denaturing characteristics of salt precipitation with the precision of HPLC to yield excellent separation of biologically active material. Waters BioSuite™ Phenyl, 1000, 10 µm HIC column media consists of a phenyl group bonded to a methacrylic ester-based polymeric resin. The large 1000 angstrom pore size accommodates proteins up to 5,000,000 Daltons. A 21.5 mm x 150 mm column is also available for "labscale" isolations.

Specifications
Chemistry: Phenyl
Separation Mode: Hydrophobic Interaction (HIC)
Particle Substrate: Polymethacrylate
pH Range Min: 2 pH
pH Range Max: 12 pH
Endcapped: Yes
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 500000
Particle Shape: Spherical
Particle Size: 13 µm
Endfitting Type: Parker-style
Pore Size: 1000 Å
Format: Column
System: HPLC
USP Classification: L11
Inner Diameter: 21.5 mm
Length: 150 mm
UNSPSC: 41115709
Application: Protein
Brand: BioSuite
Product Type: Columns
Units per Package: 1 pk