Waters, 186002560, SunFire C18 Column, 100Å, 5 µm, 4.6 mm X 250 mm, 1/pk

SunFire C18 columns are used for general purpose method development at low pH. The C18 ligand is highly retentive, especially for basic compounds and it is ideally suited for purification and impurity profile assays.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Silica
pH Range Min: 2 pH
pH Range Max: 8 pH
Maximum Pressure: 6000 psi (415 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 5 µm
Endfitting Type: Waters
Pore Size: 100 Å
Format: Column
Surface Area: 340
System: HPLC
USP Classification: L1
Inner Diameter: 4.6 mm
Length: 250 mm
Carbon Load: 16 %
UNSPSC: 41115709
Brand: SunFire
Product Type: Columns
Units per Package: 1 pk

Additional Information
FAQs about SunFire C18 Column What distinguishes the SunFire C18 Column for analytical chromatography? The SunFire C18 Column is recognized for its robustness and high-quality separations of a wide range of compounds, from small molecules to larger peptides, due to its proprietary C18 bonded phase. Is the SunFire C18 Column suitable for gradient chromatography applications? Yes, the SunFire C18 Column is designed to handle complex gradient chromatography applications, delivering sharp peaks and consistent performance over a wide range of chromatographic conditions. How does the SunFire C18 Column perform with acidic and basic compounds? The column provides excellent peak shape and resolution for both acidic and basic compounds, attributed to its stable bonding and end-capping process. Can the SunFire C18 Column be used for method development and scalability? Yes, the SunFire C18 Column is an excellent choice for method development due to its reproducibility and scalability from analytical to preparative scales. What is the recommended way to clean the SunFire C18 Column? The SunFire C18 Column should be cleaned using a series of solvent washes according to the sample matrix and stored in a recommended solvent to prevent any degradation of the bonded phase.

FAQ
What is the difference between a C18 and a C8 column? Both C8 and C18 refer to the alkyl chain of a bonded face in a column and both are used in HPLC. While they have some similarities, they are primarily different. C18 has 18 carbon atoms, while C8 has only 8, the difference that they are named for. C18 also has a longer carbon chain than C8. C18 columns will offer a longer retention and hydrophobicity than C8.
In general, C18 is used primarily in environmental science, pharmaceutical industries, radioanalytical laboratories, and chemical analysis. They are used to analyze individual parts of chemical mixtures or labeled molecules. What is HPLC? High-Performance Liquid Chromatography (HPLC) denotes an advanced analytical technique employed for the separation, identification, and quantification of constituents within a mixture. Serving as the predominant method utilized in laboratories across the globe, this test assumes paramount importance, catering to a multitude of objectives spanning various fields of research and analysis.