Waters, 186006940, ACQUITY UPLC Peptide CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 3/pk

Waters ACQUITY UPLC CSH130 C18 1.7 µm particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. The fact that the performance of a CSH130 C18 column exhibits little dependence on strong ion pairing agents makes it ideal for LC or LC-MS applications. This kit consists of three ACQUITY UPLC CSH130 C18 columns packed with three different batches of sorbent.

Specifications
Chemistry: C18
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 11 pH
Endcapped: Yes
Silanol Activity: Low
Molecular Weight Range Min: 1000
Molecular Weight Range Max: 15000
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
QC Tested: Peptide
Format: Method Validation Kit
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: CSH
USP Classification: L1
Inner Diameter: 2.1 mm
Length: 150 mm
Carbon Load: 15 %
UNSPSC: 41115709
Application: Peptide
Brand: ACQUITY UPLC
Product Type: Column Kits
Units per Package: 3 pk

Additional Information
ACQUITY UPLC Peptide CSH C18 Method Validation Kit, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 3/pk For peptides employing either FA- or TFA-containing mobile phases, Waters ACQUITY UPLC CSH130 C18 1.7 µm particles have a low and precisely controlled concentration of positive charges, producing a fairly excellent peak shape. It is suitable for LC or LC-MS applications since a CSH130 C18 column's performance shows little dependence on strong ion pairing agents. Three ACQUITY UPLC CSH130 C18 columns filled with three separate batches of sorbent make up this kit. With ACQUITY UPLC Systems, ACQUITY UPLC Columns are intended to operate without any issues. The sub-2-µm, fully porous particles technologies provide high efficiencies along with the widest sub-2-µm selectivity space. For the development of high-throughput methods, tough base-particle technologies offer best-in-class column stability and unmatched flexibility. Even at analytical mass loading, reversed-phase bonded phases frequently exhibit poor peak shape for basic chemicals when utilizing formic acid; however ACQUITY UPLC CSH Columns are an exception. These tough columns offer excellent peak shape for basic analytes when used with formic acid or other low-ionic-strength, acidic mobile phases. The long-term dependability and robustness of an analytical method are substantially impacted by the reproducibility of a chromatographic column's performance. Waters’ highly controlled batch-to-batch and column-to-column repeatability in manufacturing processes gives users of the methods confidence to keep using them. Three batches of chromatographic media (produced from various base particles) are included in the ACQUITY UPLC Method Validation Kits to test the accuracy, precision, and consistency of your procedure. The process of creating a novel chromatographic technique can be difficult and time-consuming because there are countless method parameters to experiment with. All method-development techniques are made easier by the wide range of selectivity that the UPLC Columns in our Method Development Kits cover. The broadest selection of selectivity for pH low and high technique development. Browse our website to review the full range of products offered by Waters, similar to the ACQUITY UPLC Peptide CSH C18 Method Validation Kit. You can shop for lab equipment from our website to ensure the best market price for our products. You may also want to review Cytochrome C Digestion Standard; The Cytochrome C Digestion Standard was made by digesting Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing-grade trypsin. Applications for protein digestion standards include examining the performance of mass spectrometry and chromatography apparatus as well as reviewing and benchmarking HPLC and UPLC columns.

FAQ
What Do I Need to Know About HPLC? High-performance liquid chromatography (HPLC) is an analytical technique used to separate, identify, and quantify components in a mixture. This method finds application in various fields and is the most widely employed procedure in laboratories across the globe.