Sartorius, 96IEXQ42D9MFF--A, Sartobind® Q Capsule 75 mL with 4 mm bed height

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.
Applications virus and VLP capture:
As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.
Benefits capture:
High speed purification at 5 - 30 bed volumes per minute
No size exclusion effects for large proteins an viruses / VLPs
>10fold higher binding capacities compared to conventional resins
Applications flow-through polishing:
Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.
Benefits flow-through polishing:
Virus clearance > 6 log
DNA below detection limit
Host cell protein removal > 99 %
Endotoxin removal > 5 log
Save 80 % of column costs
Easy to use:
Plug and play devices from 0.08 mL to 5 L
Can be used like a filter
Flow rate up to 30 bed volumes/min
Re-usable or disposable

Specifications
Applications:
Application: Capture; Polishing

Compliance Information:
Biological Reactivity: All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).

Connections (Physical):
Connector(s): Inlet: ¾" Sanitary | Outlet: ¾" Sanitary

Dimensions:
Bed Height: 4 mm
Dimensions WxDxH: 7.7 × 19 cm
Membrane Area: 0.27 m²

Features:
Reusable: Yes

Materials of Construction:
Housing Material: Polypropylene (PP)
Materials of Construction: Polypropylene; Polyester
Membrane Material: Stabilized reinforced cellulose

Operating Conditions:
Maximum Operating Pressure: 0.4 MPa
Maximum Venting Pressure: 0.5 bar, 0.05 MPa, 7 psi
Recommended Flow Rate: 1.5 L/min
pH Stability: pH 2 – 14 (short term), pH 2 – 12 (long term)

Physicochemical Information:
Bed Volume: 75 mL
Ligand: Quaternary ammonium
Ligand Density: 2 – 5 µeq/cm²
Pore Size: 3 – 5 µm
Reference Protein: Bovine serum albumin (BSA)
Thermal Stability: Sartobind capsules with ion exchange membranes can be autoclaved at 121 °C for 30 min. We recommend one autoclaving cycle only.
Typical Dynamic Binding Capacity 10% per unit: 2.16 g
Void Volume: 180 mL

Product Information:
Brand: Sartobind® Q
Membrane Adsorber Format: Capsules
Membrane Type: Strong anion exchange membrane
Pack Size: 4
Type: Membrane adsorber
What you receive: 4 capsules; 1 operating instruction; 1 certificate

Sterility/Purity:
Sterilization Method: Autoclavable