Sartorius, 98IEXQ42EGP, Sartobind® Q Gamma Cassette 1.6 L with 8 mm bed height

Sartobind® Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins. As size exclusion effects are neglectible, they are suited for purification of viruses such as adeno or lentiviruses displaying one order of magnitude higher dynamic binding capacity.
The cassette design eliminates the 5 L size limitation for membrane adsorbers and offers simple pod-like modular system up to 100 L membrane volume.

Sartobind® Q membrane adsorbers display a macro-porous structure with pore size of > 3 µm. Quaternary ammonium ligands are bound covalently to the complete internal surface of the membrane, resulting in separation media of high binding capacity combined with exceptional high flow rates.
Applications virus and VLP capture:
As size exclusion effects are negligible, they are suited for purification of viruses and display one order of magnitude higher dynamic binding capacity compared to resin. Viruses such as adeno- or lentiviruses are captured at high flow rates. The material allows also >100 cycles of re-use without loss of binding capacity.
Benefits capture:
High speed purification at 5 - 30 bed volumes per minute
No size exclusion effects for large proteins an viruses / VLPs
>10fold higher binding capacities compared to conventional resins
Applications flow-through polishing:
Sartobind Q is the perfect tool for the flow-through polishing of valuable proteins such as monoclonal antibodies. The bed volumes can be kept sufficiently small during the removal of viruses, DNA, host cell proteins, leached Protein A, and endotoxins from such pharmaceutical proteins.
Benefits flow-through polishing:
Virus clearance > 6 log
DNA below detection limit
Host cell protein removal > 99 %
Endotoxin removal > 5 log
Save 80 % of column costs
Easy to use:
Plug and play devices from 0.08 mL to 5 L
Can be used like a filter
Flow rate up to 30 bed volumes/min
Re-usable or disposable

Specifications
Applications:
Application: Capture; Polishing

Compliance Information:
Biological Reactivity: All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).

Connections (Physical):
Connector(s): Sanitary 1" – 1.5" via manifold

Dimensions:
Bed Height: 8 mm
Dimensions WxDxH: 634 × 49 × 387 mm
Membrane Area: 5.8 m²

Features:
Reusable: Yes

Materials of Construction:
Housing Material: Acrylonitrile butadiene styrene (ABS)
Materials of Construction: Polyester
Membrane Material: Stabilized reinforced cellulose

Operating Conditions:
Maximum Operating Pressure: 0.2 MPa
Maximum Venting Pressure: 0.5 bar, 0.05 MPa, 7 psi
Recommended Flow Rate: 8 L/min
pH Stability: pH 2 – 14 (short term), pH 2 – 12 (long term)

Physicochemical Information:
Bed Volume: 1.6 L
Chemical Compatibility: Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
Ligand: Quaternary ammonium
Ligand Density: 2 – 5 µeq/cm²
Pore Size: 3 – 5 µm
Reference Protein: Bovine serum albumin (BSA)
Thermal Stability: Sartobind cassettes with ion exchange membranes cannot be autoclaved, but they can be gamma irradiated.
Typical Dynamic Binding Capacity 10% per unit: 46 g
Void Volume: 2.9 L

Product Information:
Brand: Sartobind® Q
Membrane Adsorber Format: Cassettes
Membrane Type: Strong anion exchange membrane
Pack Size: 1
Type: Membrane adsorber
What you receive: 1 cassette; 1 operating instruction; 1 certificate

Sterility/Purity:
Sterile Validated: Yes, minimum dose for sterility is 7 – 8 kGy
Sterilization Method: Gamma irradiation

Usage Protocols:
Gamma Dose: ≥ 25 kiloGrays (kGy); maximum dose: 50 kGy