Sartorius, 98R-PA19D-L, Sartobind® Rapid A 0.8 L Cassette

The modular concept of the Sartobind® Rapid A cassettes enables larger scale purification of mAbs, while retaining direct scalability to the capsule portfolio.

Sartobind® Rapid A Cassettes integrate Sartorius agarose-based membrane functionalized with Protein A ligand in a stackable module. Using the Pilot Filter Holder, up to 13 cassettes can be connected, for a bed volume of up to 10.4 L. Despite the different formats, their performance is directly scalable to the capsule formats. Sartobind® Rapid A is engineered for time and cost-efficient mAb purification, offering competitive binding capacity (typically between 40-50 g/L1) and low pressure drop. With a simple and quick set-up and working residence times as low as 10 seconds, the overall processing time and costs can be significantly decreased. Sartobind® Rapid A Cassettes are well-suited for earlier clinical-phase and low-demand commercial manufacturing.
Features and Benefits:
Bed volume: 0.8 L/cassette
Bed-height: 4 mm
Max. operating pressure: 2 bar
Directly scalability to capsule formats
Typical flow rates: 5 – 10 MV/min2
Applications:
mAb purification
Clinical stage and lower demand commercial manufacturing
Operational Requirements:
Cassette Holder: Hydraulic clamping system for assembly and connection of the Sartobind® Rapid A cassettes.
Manifold Set: Includes the inlet and outlet plates for the Sartobind® Rapid A cassettes operation as well as the venting and draining tubing.
1dependent on the target molecule.; 2for more information please consult Sartobind® Rapid A Instructions for Use

Specifications
Applications:
Application: Capture

Compliance Information:
Biological Reactivity: The final product meets the requirements of the current ISO 10993-5 which is equivalent to the current United States Pharmacopoeia (USP) <87> Biological Reactivity Tests, in vitro.; All in-contact plastic components meet the requirements of current USP <88> Biological Reactivity Tests, in vivo, for Plastic Class VI (Systemic Injection, Intracutaneous and Implantation Tests).

Connections (Physical):
Connector(s): Sanitary 1" – 1.5"

Dimensions:
Bed Height: 4 mm
Dimensions: 634 × 49 × 387 mm

Materials of Construction:
Housing Material: Acrylonitrile butadiene styrene (ABS)

Operating Conditions:
Maximum Operating Pressure: 0.2 MPa
Maximum Venting Pressure: 0.5 bar, 0.05 MPa, 7 psi
Recommended Flow Rate: 3-5 MV/min (load, elution), 5-10 MV/min ( equilibration, wash)
pH Stability: pH 2 – 14 (short term), pH 3 – 13 (long term)

Physicochemical Information:
Bed Volume: 0.8 L
Chemical Compatibility: Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
Ligand: Protein A
Reference Protein: Human polyclonal IgG
Typical Dynamic Binding Capacity 10% per mL: ≥ 30 mg/mL
Void Volume: 2500 mL

Product Information:
Brand: Sartobind® Rapid A
Membrane Adsorber Format: Cassettes
Membrane Type: Affinity
Pack Size: 1
What you receive: 1 cassette; 1 operating instruction; 1 certificate