Sartorius, 96STPA42D4R11--A, Sartobind STIC® PA Mini 10 mL

Sartobind STIC® PA (primary amine) anion exchange membrane overcomes reduced binding capacity for host cell protein removal and can be used in high-salt conditions.

Sartobind STIC® PA removes contaminants at high salt. Salt-tolerant interaction chromatography (STIC) with PA ligand is based on anion-exchange chromatography (AEX) principles. AEX is the established method for removing process-derived contaminants such as host-cell proteins (HCP), DNA, endotoxins, and adventitious and endogenous viruses. It is performed during the downstream processing of monoclonal antibodies (mAbs) in flow-through mode. The process is driven by throughput and not by mass, and thus membrane chromatography (MC) is advantageous over columns for contaminant removal, as membranes run up to two orders of magnitude higher flow rates. Membranes are easy to operate and can be disposed of after one use, just like filters, to save on validation cost.
Applications:
Current mAb downstream processes consist of at least one initial capture and one AEX chromatography step. The limitation of AEX steps is often that the Q chemistry requires low-salt conditions. In processes with cation exchange (CEX) as the second chromatography step, the CEX pool needs to be diluted to conductivities of 4-7 mS/cm to reestablish binding conditions for the following AEX step. The Sartobind STIC PA membrane is composed of cross-linked, regenerated macro-porous cellulose. The primary amine ligand is attached to the matrix at approximately six-fold higher ligand density than that of Q membrane. Both the free amine and the high ligand density were crucial for developing its high salt tolerance.
Benefits:
The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines.
No dilution of CEX pools necessary
Host cell proteins up to 10kg/l (product load/liter membrane)
Viruses >4 log
DNA at up to 1.5 M NaCl
Endotoxins >4 log
Easy to use:
Plug and play devices from 0.08 ml to 1.6 L
Can be used like a filter
Flow rate up to 30 bed volumes/min
Disposable after one use

Specifications
Applications:
Application: Polishing

Compliance Information:
Biological Reactivity: All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).

Connections (Physical):
Connector(s): Female Luer lock

Dimensions:
Bed Height: 4 mm
Dimensions WxDxH: 70 x 54.5 cm
Membrane Area: 364 cm²

Features:
Reusable: No

Materials of Construction:
Housing Material: Polypropylene (PP)
Materials of Construction: Polypropylene; Polyester
Membrane Material: Stabilized reinforced cellulose

Operating Conditions:
Maximum Operating Pressure: 0.4 MPa
Maximum Venting Pressure: 0.5 bar, 0.05 MPa, 7 psi
Recommended Flow Rate: 200 mL/min
pH Stability: pH 2 – 14 (short term)

Physicochemical Information:
Bed Volume: 10 mL
Chemical Compatibility: Stable in common chromatography buffers, unstable to peroxide and other oxidizing or reactive reagents
Ligand: Primary amine (PA)
Ligand Density: 18 – 22 µeq/cm²
Pore Size: 3 – 5 µm
Reference Protein: Bovine serum albumin (BSA)
Thermal Stability: Sartobind® capsules with ion exchange membranes can be autoclaved at 121 °C for 30 min. We recommend one autoclaving cycle only.
Typical Dynamic Binding Capacity 10% per unit: 0.5 g
Void Volume: 32 mL

Product Information:
Brand: Sartobind® STIC PA
Membrane Adsorber Format: Capsules
Membrane Type: Salt tolerant interaction membrane (anion exchanger)
Pack Size: 4
Type: Membrane adsorber
What you receive: 4 capsules; 8 adapters Luer male to UNF 10-32 female; 1 operating instruction; 1 certificate