Abcam, ab100684, Mouse G-CSF ELISA Kit (CSF3)

Size: 1 x 96Tests
Mouse G-CSF ELISA Kit (CSF3) is a Sandwich (quantitative) ELISA for the measurement of Mouse G-CSF (CSF3) in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 0.5 pg/mL,
Range:0.62 - 150 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's G-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Mouse G-CSF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse G-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C