Abcam, ab100763, Rat ICAM1 ELISA Kit (CD54)

Size: 1 x 96Tests
Rat ICAM1 ELISA Kit (CD54) is a Sandwich (quantitative) ELISA for the measurement of Rat ICAM1 (CD54) in Rat in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:28.81 - 7000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's ICAM1 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Rat ICAM- 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Rat ICAM1 coated on a 96- well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C