Abcam, ab100915, Anti-ACOT1 antibody

Size: 100µg
Rabbit Polyclonal ACOT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT1 aa 150 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ACOT1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q86TX2

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute in 200 µL of water, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ACOT1 also known as Acyl-CoA thioesterase 1 is an enzyme involved in the hydrolysis of acyl-CoA to free fatty acid and CoA. It has a molecular mass of approximately 46 kDa. ACOT1 expresses mainly in liver kidney and heart tissues where it regulates levels of acyl-CoA an important molecule in various metabolic processes. Its activity facilitates the balance between thioesterase and transferase reactions impacting fatty acid metabolism.
Biological function summary
ACOT1 plays a role in lipid metabolism by managing the acyl-CoA pool. This enzyme helps in modulating the intracellular concentration of acyl-CoA and free fatty acids which are important for energy production and storage. It does not integrate into a larger protein complex instead functioning independently within cells. The enzyme's ability to control lipid and energy metabolism ties it to important biological processes.
Pathways
ACOT1 participates in fatty acid degradation and peroxisome proliferator-activated receptor (PPAR) signaling pathways. In these pathways ACOT1 works alongside proteins such as enzymes involved in beta-oxidation where it aids in the efficient breakdown and utilization of fatty acids. PPAR signaling influences energy balance and metabolism making ACOT1 integral to these pathways and linked with proteins like PPAR-alpha that regulate lipid metabolism.
ACOT1 has associations with metabolic syndrome and nonalcoholic fatty liver disease (NAFLD). Alterations in ACOT1 expression or activity could disrupt lipid metabolism leading to these conditions. In NAFLD changes in ACOT1 can result in excess free fatty acids affecting liver function. The enzyme connects with proteins like acetyl-CoA carboxylase which plays a part in lipid metabolism disorders demonstrating its role in these health problems.