Abcam, ab101992, Anti-HECTD1 antibody

Size: 100µg
Rabbit Polyclonal HECTD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human HECTD1 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HECTD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9ULT8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HECTD1 which stands for HECT Domain E3 Ubiquitin Protein Ligase 1 also known by other names such as KIAA1594 is an important enzyme in the protein degradation process. HECTD1 acts mechanically as an E3 ubiquitin ligase which means it helps attach ubiquitin molecules to specific protein substrates marking them for degradation by the proteasome. The protein typically weighs around 261 kDa and expresses broadly in human tissues including nervous system cardiovascular tissues and epithelial cells indicating its diverse functional roles.
Biological function summary
The enzyme HECTD1 contributes to numerous cellular processes notably protein homeostasis and cellular signaling. It acts as a part of a larger ubiquitination complex interacting with various adaptor proteins to achieve substrate specificity. By regulating the fate of proteins within the cell HECTD1 impacts processes like cell cycle progression apoptosis and stress responses. Studies show that it also plays a role in embryonic development pointing to its function in tissue morphogenesis and organogenesis.
Pathways
HECTD1 has significant roles within the ubiquitin-proteasome pathway and the cell cycle regulatory pathway. It interacts with proteins such as SMAD2 in the TGF-beta signaling pathway which controls cell proliferation and differentiation. This enzyme also coordinates with TRAF6 in immune signaling pathways influencing immune responses. These interactions highlight HECTD1's pivotal role in maintaining cellular function by modulating protein stability and activity.
Mutations or deregulation of HECTD1 associate with neurodevelopmental disorders and cancer. Abnormal HECTD1 activity can disrupt normal brain development linking it to certain neurodevelopmental disorders. In cancer altered expression or function of HECTD1 affects cell proliferation pathways leading to uncontrolled growth. Researchers find HECTD1 interacting with proteins like c-MYC in oncogenic pathways further establishing its relevance in the pathophysiology of human diseases.