Abcam, ab10260, Anti-ERAB antibody [5F3]

Size: 100µL
Mouse Monoclonal ERAB antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSD17B10.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:5F3,
Isotype:IgG1,
Light chain type:lambda,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human HSD17B10.Q99714,
Specificity:This antibody detects the recombinant human ERAB protein (27kDa) and recognizes the endogenous ERAB protein in cell extracts with virtually no crossreactivity with other human proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Ammonium sulfate precipitated and dialyzed tissue culture supernatant., Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERAB also known as 17-beta-hydroxysteroid dehydrogenase type 10 (HSD10) or 3-hydroxyacyl-CoA dehydrogenase type II is a multifunctional mitochondrial enzyme. It has a molecular mass of approximately 27 kDa. This enzyme is expressed in various tissues with high expression in the liver kidney heart and brain. ERAB plays a role in several metabolic processes thanks to its ability to catalyze reactions involving the oxidation of hydroxyacyl-CoA to ketoacyl-CoA derivatives alongside other substrates like steroids.
Biological function summary
The function of this enzyme extends beyond its metabolic activities. ERAB is part of the mitochondrial enzyme complex responsible for fatty acid oxidation and energy production. This places it centrally in cellular energy homeostasis. Additionally ERAB participates in the metabolism of neurosteroids which impacts brain function. Through its interactions ERAB influences signaling pathways by modulating local steroid concentrations in the nervous system affecting cellular communication and response.
Pathways
ERAB associates with key biochemical pathways including the beta-oxidation of fatty acids and the steroid hormone metabolism pathway. It interacts with acetyl-CoA acyltransferase 2 (ACAA2) within these metabolic pathways facilitating the conversion of substrates vital for energy production and steroid synthesis. This involvement underpins the enzyme’s significance in maintaining metabolic equilibrium through its catalytic activities on essential biological molecules.
ERAB has links to Alzheimer's disease and rare mitochondrial disorders. Studies show that ERAB binds amyloid-beta peptides which accumulate in the brains of Alzheimer's disease patients. This interaction suggests ERAB's potential contribution to the pathogenesis of the disease. Additionally mutations affecting ERAB lead to HSD10 mitochondrial disease a disorder characterized by metabolic and neurological defects. One of the proteins that interacts with ERAB in the context of disease is amyloid precursor protein (APP) which further implicates ERAB in Alzheimer's disease through pathological pathways.