Abcam, ab104224, Anti-NeuN antibody [1B7] - Neuronal Marker

Size: 100µg
Anti-NeuN antibody [1B7] - Neuronal Marker (ab104224) is a mouse monoclonal antibody detecting NeuN in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 620 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1B7,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RBFOX3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.A6NFN3

Product details:
Anti-NeuN antibody [1B7] - Neuronal Marker (ab104224) is a mouse monoclonal antibody and is validated for use in ICC/IF, IHC-P and WB.
Anti-NeuN antibody [1B7] - Neuronal Marker (ab104224) was first used in a scientific publication in 2013 and has been cited over 621 times in peer reviewed journals. It's performance in Western blot, immunofluorescence and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-NeuN antibody [1B7] - Neuronal Marker (ab104224) has high sensitivity and specificity.
Anti-NeuN antibody [1B7] - Neuronal Marker (ab104224) has 26 independent reviews from customers.
Anti-NeuN antibody [1B7] - Neuronal Marker (ab104224) specifically detects NeuN (UniProt ID: A6NFN3; Molecular weight: 34kDa) and is sold in 100 ug selling sizes.
NeuN is a widely used marker in neuro research for identifying mature neurons. It is expressed in the nuclei and, to a lesser extent, the cytoplasm of post-mitotic neurons. Researchers use NeuN to assess neuronal density and integrity, making it essential for studying neuronal development, neurodegenerative diseases and brain injuries. Its reliable expression in mature neurons helps in evaluating neuronal loss and therapeutic effects in various neurological conditions.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.03% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NeuN also known as Fox-3 is a protein often utilized as a neuronal marker due to its expression predominantly in neurons. The molecular weight of NeuN is approximately 46-48 kDa. It is located in the nucleus and in the post-mitotic neurons of the central nervous system. Research frequently uses anti-NeuN antibodies for identifying neuronal cells in various samples capitalizing on its reliable expression profile across diverse neuron types. Common techniques for detecting NeuN include Western blot and immunohistochemistry (IHC) with NeuN staining being a definitive method for confirming neuronal identity.
Biological function summary
NeuN plays a role in RNA splicing specific to neurons contributing to the regulation of gene expression within the nervous system. It does not function as part of a larger protein complex but interacts with chromatin to influence neuronal gene expression. This has implications for neuronal differentiation and maintenance. Techniques like NeuN IHC use antibodies such as Alexa Fluor 568 conjugated variants to visualize neurons in tissue confirming its utility as an accessible and specific neuronal marker in research.
Pathways
NeuN serves an important function in neuronal development and differentiation. It particularly influences neurogenesis and synaptogenesis pathways where neuron-specific splicing and transcriptional regulation are essential. Although NeuN does not directly engage in pathways with other proteins because it operates independently its expression parallels the activity of significant neuronal proteins such as MAP2 and Neurofilaments. These relationships illustrate NeuN's indirect association in pathways promoting neuronal development and maintenance.
NeuN remains significant in studies of neurodegenerative diseases and neuronal damage. For instance decreased NeuN staining has been observed in Alzheimer's disease indicating loss of neuronal identity or survival. Research also notes alterations in NeuN expression following ischemic injury where its presence or absence links to neuronal viability. NeuN's association with these states of neuronal health or pathology highlights its relevance as a marker in neurodegenerative and injury-related disorders. Furthermore while not directly in disease pathways NeuN functionally intersects with proteins like Tau in Alzheimer's suggesting its indirect role in disease pathways.