Abcam, ab105157, PE Anti-Neutrophil antibody [7/4]

Size: 50Test
Rat Monoclonal Neutrophil antibody - conjugated to PE. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing Neutrophil protein.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:7/4,
Isotype:IgG2a,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:Recognizes the Ly-6B.2 antigen.,
Specificity:Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.

Product details:
ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells
in vivo.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neutrophils also known as polymorphonuclear leukocytes are a type of white blood cell essential in the immune response. These cells make up around 50-70% of the total white blood cells in human circulation. Neutrophils express various surface markers including Ly6B.2 and NIMP-R14 which are important for their identification. The typical lifespan of neutrophils is short about 6 to 24 hours and they have an approximate mass of 1.4 x 10^-12 g per cell. Neutrophils primarily reside in the bloodstream but can migrate into tissues during inflammation or infection.
Biological function summary
Neutrophils play a central role in innate immunity by engulfing and destroying pathogens through phagocytosis. They exist as part of a network with other immune cells and contribute to the release of enzymes and reactive oxygen species to combat infection. Neutrophils can also secrete granules containing antimicrobial substances. Their activity is coordinated with other components of the immune system to provide an immediate response to infections and tissue injuries.
Pathways
Neutrophils participate actively in inflammatory and immune responses. They are important in pathways like the leukocyte adhesion cascade and the oxidative burst pathway. These pathways involve interactions with proteins such as integrins and NADPH oxidase which help neutrophils adhere to endothelium and generate reactive oxygen species respectively. These interactions permit neutrophils to migrate to sites of inflammation and eliminate invading pathogens efficiently.
Neutrophils relate to conditions such as chronic granulomatous disease and rheumatoid arthritis. In chronic granulomatous disease defects in components like NADPH oxidase result in an impaired ability to produce reactive oxygen species leading to recurrent infections. In rheumatoid arthritis neutrophils contribute to inflammation and joint damage through the release of enzymes and reactive oxygen intermediates. Neutrophil interactions with immune complexes in these disorders highlight the importance of their regulation in maintaining immune homeostasis.