Abcam, ab106174, Anti-SIGLEC H antibody [440c]

Size: 50µg
Rat Monoclonal SIGLEC H antibody. Suitable for Flow Cyt, FuncS (Neut/Block), IHC-Fr and reacts with Mouse samples. Cited in 2 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:440c,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse,
Applications:Flow Cyt, FuncS (Neut/Block), IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-ab106174 was 0.2 µm filtered prior to formulation., Storage buffer-Constituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIGLEC H also known as sialic acid-binding Ig-like lectin H weighs about 50 kDa. It is found on the surface of plasmacytoid dendritic cells (pDCs) and it is closely related to the immune system. This protein lacks a signaling domain but it can pair with other proteins to transmit signals across cell membranes. The expression of SIGLEC H is highly specific to pDCs making it a useful marker for these cells in various immunological studies.
Biological function summary
This protein plays an important role in the immune response. SIGLEC H functions in the recognition and uptake of pathogens through endocytosis by binding sialylated glycoproteins. Unlike some other members of the SIGLEC family it is not part of a complex and relies on interactions with other signaling molecules. These interactions facilitate phagocytosis and antigen presentation which are critical functions of pDCs in adaptive immunity.
Pathways
SIGLEC H becomes involved in the innate immune signaling pathways that regulate type I interferon production. The pathways include the Toll-like receptor (TLR) signaling pathway. SIGLEC H modulates TLR7 and TLR9 pathways in pDCs which manage the production of cytokines and chemokines. It can also influence the expression of interferon regulatory factors further impacting cytokine production and modulating immune response.
The improper function or regulation of SIGLEC H affects autoimmune diseases like systemic lupus erythematosus. Anomalies in SIGLEC H might also have implications for viral infections due to its role in pathogen detection and immune responses. Proteins such as TLR7 and interferon regulatory factor 7 (IRF7) show connections with SIGLEC H within these contexts often determining the outcome of immune-mediated and infectious diseases.