Abcam, ab106555, Anti-RBM35A + RBM35B antibody [23A7.C9]

Size: 100µg
Mouse Monoclonal ESRP1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Esrp1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:23A7.C9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Mouse Esrp1.Q3US41,
Specificity:This antibody reacts with both mouse Esrp-1 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-ab106555 was purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered., Storage buffer-pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RBM35A and RBM35B also known as ESRP1 and ESRP2 are RNA-binding proteins that play roles in the regulation of alternative splicing. ESRP1 has a mass of approximately 75 kDa while ESRP2 is slightly larger. These proteins are expressed widely in epithelial tissues. They bind specific RNA sequences influencing exon inclusion or exclusion during pre-mRNA splicing. This action alters the outcome and diversity of proteins produced from the gene impacting various cellular processes.
Biological function summary
RBM35A and RBM35B impact the post-transcriptional regulation of gene expression. They often function as part of larger spliceosomal complexes. By deciding the splicing pattern of mRNA they contribute to the diversity of the proteome especially in epithelial cell types. This diversity is important for cell differentiation and tissue-specific functions. The proteins influence the expression of genes involved in cellular adhesion and signaling pathways which are critical for maintaining epithelial cell features.
Pathways
RBM35A and RBM35B play roles in the epithelial-mesenchymal transition (EMT) and Notch signaling pathways. During EMT these proteins regulate key splicing events that change cell adhesion and mobility. This involves interactions with proteins such as FGFR2 and CD44 which play roles in cellular communication and structure. In the Notch signaling pathway RBM35A and RBM35B influence cell fate decisions through alternative splicing of pathway components.
RBM35A and RBM35B have implications in cancer and fibrosis. Their altered expression or function can contribute to cancer progression by influencing EMT a pathway linked to metastasis. Changes in their activity associate with fibrotic diseases where excessive tissue scarring occurs. The interaction of these proteins with CD44 which is involved in tumor progression and fibrosis highlights their role in these processes.