Abcam, ab108727, Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit

Size: 1 x 96Tests
Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Corynebacterium diphtheriae toxin in human plasma and serum samples. - Colorimetric readout - 450 nm - Works on any standard plate reader - Easy results interpretation - Cut-off, positive and negative controls included
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Sensitivity:= 0.01 IU/mL,
Range:0.01 - 1 IU/mL,
Assay Platform:Microplate

Product details:
Abcam's anti-Corynebacterium diphtheriae toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Corynebacterium diphtheriae toxin in Human serum and plasma.
A 96-well plate has been precoated with Corynebacterium diphtheriae toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Corynebacterium diphtheriae toxin 5S-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Corynebacterium diphtheriae toxin IgG sample captured in plate.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C