Abcam, ab108899, Human Retinol binding protein ELISA Kit (RBP)

Size: 1 x 96Tests
Human Retinol binding protein ELISA Kit (RBP) is a Sandwich (quantitative) ELISA for the measurement of Human Retinol binding protein (RBP) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Cell culture supernatant, Milk,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.0078 µg/mL,
Range:0.008 - 0.5 µg/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Abcam's Retinol binding protein (RBP) Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein concentrations in Human milk, urine, CSF, saliva and cell culture supernatants.
A Retinol binding protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Retinol Binding Protein (RBP) also known as RBP4 is a small transport protein with a molecular weight of approximately 21 kDa. It plays an essential role in the transport of vitamin A (retinol) throughout the body. RBP expresses mainly in the liver and is released into the bloodstream to carry retinol to various organs. This protein binds with retinol to form a complex that enables safe transportation as retinol is hydrophobic and insoluble in water.
Biological function summary
RBP interacts with the cell membrane receptors to deliver retinol to target cells where it's needed for various physiological processes. It functions either independently or as part of a complex with transthyretin (TTR) in the extracellular space. This interaction with TTR stabilizes RBP and its retinol cargo in circulation and prevents renal clearance. The presence of this complex is significant for maintaining vitamin A homeostasis in the body.
Pathways
RBP serves an important role in retinoid metabolism and transport pathways. In the retinoid pathway RBP ensures the bioavailability of retinol which is further metabolized into active metabolites like retinal and retinoic acid. These metabolites are important for vision and gene regulation. RBP works closely with proteins such as STRA6 (Stimulated by Retinoic Acid Gene 6) a membrane receptor that facilitates retinol uptake into cells further connecting RBP to cellular retinoic acid signaling pathways.
RBP4 has associations with conditions like type 2 diabetes and obesity. Elevated levels of RBP4 in the bloodstream have been linked to insulin resistance a common feature of type 2 diabetes. Studies suggest that these high levels of RBP4 might interfere with insulin signaling contributing to the disease pathogenesis. Another protein of interest in these disorders is adiponectin which inversely correlates with RBP4 levels; as RBP4 increases adiponectin tends to decrease highlighting a potential mechanism by which RBP4 may impact metabolic diseases.