Abcam, ab108913, Rat TNF alpha ELISA Kit

Size: 1 x 96Tests
Rat TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat TNF alpha in Rat in Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.0082 ng/mL,
Range:0.0156 - 1 ng/mL,
Assay time:4h 30m,
Assay Platform:Microplate

Product details:
Abcam's TNF alpha Rat
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in rat cell culture supernatants.
A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha X specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols