Abcam, ab109912, Monoamine oxidase B Activity Assay Kit ((MAOB Assay)

Size: 1 x 96Tests
ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Tissue Extracts,
Reacts with:Human,
Assay type:Enzyme activity,
Assay Platform:Microplate reader

Product details:
ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated MAOB is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
Store Fluorophore and benzylamine at -80°C. Store all other components store at 4°C.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Monoamine Oxidase B (MAO-B) also known as MAOB or MAO-B inhibitor is an enzyme that is a part of the flavin-containing amine oxidases group. It exists as a homodimer with each monomer having a mass of about 58 kDa. This enzyme plays an important role in the catabolism of monoamines by oxidizing neurotransmitters such as dopamine in the brain. MAO-B is expressed mainly in the outer membrane of mitochondria in neuronal and glial cells and to a lesser extent in other tissues like the liver.
Biological function summary
The enzyme contributes to the breakdown of biogenic and dietary amines supporting neurotransmitter regulation and signal modulation. It does not work as part of a larger protein complex but has important interactions within its local cellular environment. MAO-B helps maintain the proper levels of monoamine neurotransmitters preventing their accumulation and potential neurotoxicity.
Pathways
Monoamine Oxidase B functions in the dopamine and serotonin metabolic pathways where it actively participates in the oxidative deamination of monoamines. In these pathways MAO-B acts alongside other proteins such as Monoamine Oxidase A (MAO-A) which shares similar functions and substrate preferences. This enzyme activity helps modulate mood and emotional behaviors which are critical for normal brain function.
Monoamine Oxidase B has a significant connection with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. In Parkinson's disease MAO-B intensifies the oxidative stress by promoting dopamine breakdown which is linked to neuronal degeneration. In Alzheimer's disease elevated MAO-B activity may contribute to the overproduction of neurotoxic metabolites. Through these conditions MAO-B is often targeted by MAO-B inhibitors to reduce its activity alleviating symptoms and slowing disease progression.