Abcam, ab110216, MitoBiogenesis™ In-Cell ELISA Kit (IR)

Size: 2 x 96Tests
MitoBiogenesis™ In-Cell ELISA Kit (IR) is a Cell-based (quantitative) ELISA for the measurement of MitoBiogenesis™ In-Cell (IR) in in Cell Samples samples from a wide range of species.
Key facts
Detection method:IR,
Sample types:Adherent cells,
Reacts with:Mouse, Rat, Cow, Human,
Assay type:Cell-based (quantitative),
Assay Platform:Microplate

Product details:
For identifying inhibitors and activators of mitochondrial biogenesis in adherent cultured cells. Each kit contains sufficient reagents to analyze two 96-well plates of fixed human, rat, mouse, or bovine cells. This kit utilizes IRDyes® for detection, and so requires a LI-COR® Odyssey® or Aerius® imaging system. An alternate colorimetric version of this kit is available for use with standard plate readers - MitoBiogenesis™ In-Cell ELISA Kit (Colorimetric) (
ab110217
/MS643).
In-Cell ELISA Kits use quantitative immunocytochemistry to measure protein levels or post-translational modifications in cultured cells. Cells are fixed in a 96-well plate and targets of interest are detected with highly-specific, well-characterized monoclonal antibodies, and levels are quantified with IRDye®-labeled Secondary Antibodies. IR imaging and quantitation is performed using a LI-COR® Odyssey® or Aerius® system.
Cells (human, rat or mouse) are seeded in 96- or 384-well microplates, and after exposure to experimental compounds for several cell doublings, the levels of two mitochondrial proteins are measured simultaneously in each well. The two proteins are each subunits of a different oxidative phosphorylation enzyme complex, one protein being subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the other being the 70 kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. Optionally, total protein levels can also be measured.
LI-COR®, Odyssey®, Aerius®, IRDye™ and In-Cell Western™ are registered trademarks or trademarks of LI-COR Biosciences Inc
Plates are available in our ICE (In-Cell ELISA) Support Pack (
ab111542
) which can be bought separately
**Related products** Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C