Abcam, ab110329, Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker

Size: 100µg
Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) is a mouse monoclonal antibody detecting Mitofilin in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Cow, Human, Mouse, Rat . - Over 50 publications
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2E4AD5,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Cow, Human,
Applications:IHC-P, Flow Cyt, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What is this antibody validated in?
Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (ab110329) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.
What is the molecular weight of Mitofilin?
Anti-Mitofilin [2E4AD5] - Mitochondrial Marker (ab110329) specifically detects a band for Mitofilin (UniProt: Q16891) at a molecular weight of 84kDa.
Trusted by the scientific community
Anti-Mitofilin [2E4AD5] - Mitochondrial Marker (ab110329) was first used in a scientific publication in 2011 and has been cited over 50 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [2E4AD5] also available for your convenience: ab110329, Alexa Fluor® 488 -
ab198028
, Alexa Fluor® 647 -
ab198036
, HRP -
ab198037
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification notes-ab110329 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.Purity: >95% by SDS-PAGE., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mitofilin also known as mitofilin or MIM is a protein with a mass of approximately 88 kDa. This protein is integral to the inner mitochondrial membrane. Mitofilin is found in various tissues with high expression in the heart and skeletal muscle indicating its significant role in energy-demanding tissues. It is the central component of the mitochondrial inner membrane organizing system (MINOS) which plays a fundamental role in maintaining the mitochondrial architecture.
Biological function summary
Within the mitochondrial environment mitofilin contributes to maintaining cristae morphology which is essential for optimal mitochondrial respiration and energy production. As part of the MINOS complex mitofilin interacts with other proteins such as Mic60 and Mic10 stabilizing the structure of crista junctions. This process ensures efficient electron transport chain function ultimately supporting ATP synthesis. Without effective shaping of the cristae cellular energy metabolism deteriorates.
Pathways
Research has highlighted the importance of mitofilin in apoptosis and bioenergetics pathways. It interacts with proteins like OPA1 a dynamin-related GTPase important for mitochondrial fusion and energy metabolism. Additionally mitofilin influences the release of cytochrome c an important step in the initiation of apoptosis. Proper functioning of these pathways is critical for cellular balance and survival showing mitofilin's broad impact on cellular processes.
Mitofilin has links to mitochondrial myopathies and cardiomyopathies. These disorders stem from impaired mitochondrial function significantly affecting muscle tissues and heart performance. Disruption in mitofilin expression or function can lead to compromised energy production contributing to these conditions. Furthermore mitofilin has associations with proteins like Drp1 involved in mitochondrial dynamics and this relationship can influence the progression and severity of mitochondrial-related diseases.