Abcam, ab111309, Anti-4E-BP2 antibody

Size: 50µg
Rabbit Polyclonal 4E-BP2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic translation initiation factor 4E-binding protein 2 aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Eukaryotic translation initiation factor 4E-binding protein 2 aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.Q13542,
Specificity:Detects 17 and 20 kD proteins, corresponding to the apparent molecular mass of PHAS II and its phosphorylated state on SDS-PAGE immunoblots.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 8.4 Constituents: 0.44% Sodium chloride, 0.15% EDTA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 4E-BP2 protein also known as eukaryotic translation initiation factor 4E-binding protein 2 is an important regulator of translation initiation. It is a small protein with a molecular weight of approximately 14 kDa. 4E-BP2 is expressed in various tissues with a prominent presence in the brain. This protein binds to eukaryotic initiation factor 4E (eIF4E) inhibiting the initiation of cap-dependent translation by preventing eIF4E from assembling into the larger eIF4F complex.
Biological function summary
The 4E-BP2 protein inhibits the initiation of protein synthesis regulating gene expression at a translational level. 4E-BP2 does not form part of a stable complex but transiently interacts with eIF4E in response to different cellular signals. When 4E-BP2 is phosphorylated by mammalian target of rapamycin (mTOR) it undergoes a conformational change that weakens its binding to eIF4E allowing protein synthesis to proceed. This regulation exhibits tissue specificity and involves responses to growth factors nutrients and cellular stress.
Pathways
The involvement of 4E-BP2 is important in the mTOR signaling pathway a central pathway regulating cell growth and metabolism in response to environmental cues. The mTOR pathway includes other proteins like eIF4E which 4E-BP2 interacts with directly and mTOR itself which phosphorylates 4E-BP2. Additionally 4E-BP2 also participates in the insulin signaling pathway influencing how cells respond to insulin and other growth factors by regulating protein translation.
Altered 4E-BP2 function has implications in neurological disorders and cancer. In the brain dysregulation of 4E-BP2 is associated with autism spectrum disorders as its role in neural development and plasticity is important. In cancer aberrant activity of 4E-BP2 can lead to unchecked cellular proliferation due to disrupted control of protein synthesis. The protein's regulation by mTOR links it to various cancers as increased mTOR activity can result in decreased 4E-BP2 activity facilitating tumorigenesis.