Abcam, ab11132, Anti-beta Amyloid antibody [DE2B4]

Size: 50µg
Anti-beta Amyloid antibody [DE2B4] (ab11132) is a mouse monoclonal antibody detecting beta Amyloid in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Human . - Over 30 publications - Trusted since 2004
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:DE2B4,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, ICC/IF, IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human APP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P05067,
Epitope:ab11132 recognizes an epitope in the region 8-16 of human beta Amyloid.

Product details:
What is this antibody validated in?
Anti-beta Amyloid antibody [DE2B4] (ab11132) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of beta Amyloid?
Anti-beta Amyloid [DE2B4] (ab11132) specifically detects a band for beta Amyloid (UniProt: P05067) at a molecular weight of 87kDa.
Trusted by the scientific community
Anti-beta Amyloid [DE2B4] (ab11132) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-beta Amyloid [DE2B4] (ab11132) has over 5 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [DE2B4] also available for your convenience: ab11132, HRP -
ab187908

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beta amyloid also known as amyloid beta peptide is a small protein fragment composed of 36-43 amino acids. It typically has a mass of approximately 4 kDa. This peptide primarily emerges from the amyloid precursor protein (APP) through enzymatic actions by beta-secretase and gamma-secretase. Beta amyloid is commonly expressed in the brain particularly within the neuronal tissue. It is known for aggregating into insoluble fibrils leading to the formation of beta amyloid plaques. Researchers often study beta amyloid using tools like amyloid beta IHC and with specific antibodies like moab 2 and 2e9 which help in detecting its presence and distribution.
Biological function summary
In the context of neuronal function beta amyloid is significant yet contentious. It is believed to play a role in synaptic transmission and may partake in homeostatic regulation. However the true physiological role still remains not well defined. Beta amyloid often self-assembles into oligomers and further into beta amyloid plaques which are part of a larger complex that includes various cellular and molecular components. The plaques contribute to neural pathway disruptions and may interfere with synaptic connections.
Pathways
Beta amyloid integrates into important cellular processes such as the amyloidogenic and non-amyloidogenic pathways. The amyloidogenic pathway involves the sequential cleavage of APP by beta and gamma secretases leading to beta amyloid release which can aggregate. In contrast the non-amyloidogenic pathway mediated by alpha-secretase precludes beta amyloid formation. Proteins such as presenilin-1 and nicastrin are closely tied to beta amyloid formation due to their roles in the gamma-secretase complex.
Beta amyloid is primarily associated with Alzheimer's disease where its accumulations form characteristic amyloid plaques observed in patients' brains. These plaques are implicated in neuronal damage and cognitive decline. Beyond Alzheimer's beta amyloid may also connect to cerebral amyloid angiopathy a condition marked by amyloid deposits in the blood vessels of the brain leading to increased risk of hemorrhagic stroke. Recent studies suggest other proteins such as tau link closely with beta amyloid pathology in Alzheimer's promoting neurofibrillary tangles and synaptic degeneration.