Abcam, ab113202, Anti-NET1 antibody

Size: 100µg
Rabbit Polyclonal NET1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human NET1 aa 500 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NET1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q7Z628

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NET1 or Neuroepithelial Transforming Gene 1 is a fascinating protein often identified by scientists because of its role in cellular dynamics. NET1 weighs approximately 64 kDa and functions mainly as a guanine nucleotide exchange factor (GEF) for RhoA a small GTPase. NET1 facilitates the conversion of inactive GDP-bound RhoA into its active GTP-bound form. NET1 prominently expresses in several tissues including the liver and kidneys. Researchers often focus on NET1's mechanical action in connection to its influence on the cytoskeleton and cellular movement.
Biological function summary
The primary function of NET1 revolves around its ability to regulate RhoA activity significantly impacting cell shape and mobility. NET1 plays a role in actin cytoskeleton reorganization making it important to processes such as cell migration and adhesion. NET1 acts within a protein complex that includes RhoA and other neighboring molecules like GTPase-activating proteins and guanine nucleotide dissociation inhibitors. This interaction highlights its importance for cellular structures and functions connected to movement and stability.
Pathways
NET1 engages with cellular pathways controlling cytoskeleton organization and cellular migration. It prominently participates in the Rho signaling pathway a critical regulator of the actin cytoskeleton. NET1's activity in these pathways associates closely with proteins such as ROCK a Rho-associated kinase that influences cytoskeletal rearrangements. The connection of NET1 with focal adhesion pathways showcases its effective role in anchoring cells to their surroundings and responding to mechanical cues.
NET1 exhibits significant links with cancer and fibrosis. NET1 overexpression correlates with increased invasiveness and metastatic potential in various cancer types including breast and gastric cancer. Researchers note NET1's connection to epithelial-mesenchymal transition (EMT) a process vital for cancer progression. Furthermore NET1 involvement extends to fibrotic disorders where excessive connective tissue forms. In these diseases NET1 interacts with fibrotic proteins like collagen indicating its contribution to pathological tissue remodeling.