Abcam, ab113350, Rat IL-1 alpha ELISA Kit

Size: 1 x 96Tests
Rat IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-1 alpha in Rat in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:< 15 pg/mL,
Range:11 - 8000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's IL-1 alpha Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL- 1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C