Abcam, ab114372, Recombinant Human Prosurfactant Protein B

Size: 10µg
Recombinant Human Prosurfactant Protein B is a Human Full Length protein, in the 1 to 381 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.
Key facts
Expression system:Wheat germ,
Tags:GST tag N-Terminus,
Applications:WB, ELISA, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P07988,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 0.79% Tris HCl, 0.3% Glutathione

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prosurfactant Protein B also known as surfactant protein B (SP-B) is essential for pulmonary function. It is a hydrophobic protein with an approximate mass of 8 kDa after processing from its precursor form which is larger. The protein is expressed predominantly in type II alveolar cells in the lungs and is found in lamellar bodies. SP-B functions mechanically by reducing surface tension in the alveoli facilitating efficient gaseous exchange and lung compliance.
Biological function summary
SP-B plays a significant role in the formation and stabilization of pulmonary surfactant film. It acts in conjunction with phospholipids to form a protein-lipid complex. This complex is critical for lowering surface tension at the air-liquid interface in the alveoli preventing lung collapse during exhalation. SP-B integrates lipid components into the surfactant matrix and enhances its spreading and surface activity.
Pathways
SP-B serves an integral part in lung surfactant metabolism and the phospholipid metabolic pathway. These pathways ensure the homeostasis and turnover of surfactant materials. Proteins such as surfactant proteins A (SP-A) and C (SP-C) are also involved in these pathways coordinating with SP-B to maintain surfactant function and lung physiology. Together these proteins help maintain the balance necessary for proper respiratory mechanics.
Deficiency or mutation in SP-B is associated with respiratory distress syndrome (RDS) and interstitial lung diseases. These conditions manifest due to compromised surfactant function leading to alveolar collapse. SP-B mutations can disrupt the normal surfactant pathway leading to insufficient lung function. Disorders like these often involve SP-C as well with both proteins affected in cases of congenital surfactant deficiency further impacting pulmonary health.